Journal: ACS applied materials & interfaces

Article Title: Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging

doi: 10.1021/acsami.7b14362

Figure Lengend Snippet: (a) Chemical structure of the αMSH peptide. (b) HPLC trace of the purified αMSH peptide sequence. (c) IC50 determination of the MC1-R targeting D-Lys-ReCCMSH peptide.

Article Snippet: General Synthesis procedures of Peptides Three MC1-R targeting ReCCMSH peptide analogs ( Figure S1 ) were synthesized using standard fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide chemistry on a multiple peptide synthesizer (Model 396, AAPPTEC or Tetras, Advanced ChemTech).

Techniques: Purification, Sequencing

Journal: ACS applied materials & interfaces

Article Title: Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging

doi: 10.1021/acsami.7b14362

Figure Lengend Snippet: Cellular binding/uptake of M21 and B16F10 cells as a function of particle concentration (a) and incubation time (b) by flow cytometry. Percentage of the total events detected is displayed. Each data point represents mean±s.d. of three replicates. (c–e) Specific binding of αMSH-PEG-Cy5-C′ dots. (c, d) MC1-R blocking of M21 and B16F10 cells, respectively, using flow cytometry and anti-MC1-R antibody prior to the addition of targeted C′ dots. (e) Tumor-directed binding of αMSH-PEG-Cy5-C′ dots in B16F10 cells with one targeted particle concentration (25 nM) versus scrambled peptide-bound particle control, assayed by flow cytometry. Each bar represents mean±s.d. of three replicates. (f) Competitive MC1-R binding studies of aMSH-PEG-C’dots and Scr-αMSH-PEG-Cy5-C′ dots over a concentration range of 10−13 to 10−5 mol/L with 125I-NDP in B16/F10 melanoma cells. Each data point reflects mean±s.d. of three replicates.

Article Snippet: General Synthesis procedures of Peptides Three MC1-R targeting ReCCMSH peptide analogs ( Figure S1 ) were synthesized using standard fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide chemistry on a multiple peptide synthesizer (Model 396, AAPPTEC or Tetras, Advanced ChemTech).

Techniques: Binding Assay, Concentration Assay, Incubation, Flow Cytometry, Blocking Assay

Journal: ACS applied materials & interfaces

Article Title: Melanocortin-1 Receptor-Targeting Ultrasmall Silica Nanoparticles for Dual-Modality Human Melanoma Imaging

doi: 10.1021/acsami.7b14362

Figure Lengend Snippet: at 2, 24, 48 and 72 h post-injection of the MC1-R-targeting particle tracer into two cohorts of B16F10 xenografted mice: (a) targeted group and (b) NDP blocking group. For the blocking study, each mouse was co-injected with 89Zr-DFO-αMSH-PEG-Cy5-C′ dots and 200 μg of NDP. N=3 for each group.

Article Snippet: General Synthesis procedures of Peptides Three MC1-R targeting ReCCMSH peptide analogs ( Figure S1 ) were synthesized using standard fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide chemistry on a multiple peptide synthesizer (Model 396, AAPPTEC or Tetras, Advanced ChemTech).

Techniques: Injection, Blocking Assay